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GSNOR,ADH5 Polyclonal antibody

GSNOR,ADH5 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 16379-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), IP, ELISA

ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存


经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue, human liver tissue, human testis tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16379-1-AP targets GSNOR,ADH5 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 GSNOR,ADH5 fusion protein Ag9360 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alcohol dehydrogenase 5 (class III), chi polypeptide
别名 ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5
计算分子量 374 aa, 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC014665
基因名称 ADH5
Gene ID (NCBI) 128
RRIDAB_2289298
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11766
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

实验方案

Product Specific Protocols
WB protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
IHC protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
IF protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
IP protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
FC protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Gene Copy Number and Post-Transductional Mechanisms Regulate TRAP1 Expression in Human Colorectal Carcinomas.

Authors - Michele Pietrafesa
  • KD Validated
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