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  • Featured Product
  • KD/KO Validated

GSNOR,ADH5 Polyclonal antibody

GSNOR,ADH5 Polyclonal Antibody for FC, IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16379-1-AP

Print datasheet

Synonyms

ADH 3, ADH5, ADHX, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, FALDH, FDH, GSH FDH, GSNOR, GSNOR,ADH5

验证数据展示

  • WB analysis of mouse liver using 16379-1-AP

    WB analysis of mouse liver using 16379-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 16379-1-AP

    WB analysis of HepG2 using 16379-1-AP

    WB result of GSNOR,ADH5 antibody (16379-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells.

  • IP experiment of mouse liver using 16379-1-AP

    IP experiment of mouse liver using 16379-1-AP

    IP result of anti-GSNOR,ADH5 (IP:16379-1-AP, 3ug; Detection:16379-1-AP 1:800) with mouse liver tissue lysate 4000ug.

  • IF Staining of HepG2 using 16379-1-AP

    IF Staining of HepG2 using 16379-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 16379-1-AP

    FC experiment of HepG2 using 16379-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug GSNOR,ADH5 antibody (16379-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

经过测试的应用

Positive WB detected inmouse liver tissue, human liver tissue, human brain tissue, human testis tissue, mouse heart tissue, HepG2 cells, mouse brain tissue
Positive IP detected inmouse liver tissue
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16379-1-AP targets GSNOR,ADH5 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GSNOR,ADH5 fusion protein Ag9360 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alcohol dehydrogenase 5 (class III), chi polypeptide
Synonyms ADH 3, ADH5, ADHX, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, FALDH, FDH, GSH FDH, GSNOR, GSNOR,ADH5
Calculated Molecular Weight 374 aa, 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC014665
Gene Symbol ADH5
Gene ID (NCBI) 128
RRIDAB_2289298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11766
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

实验方案

Product Specific Protocols
WB protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
IHC protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
IF protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
IP protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
FC protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Gene Copy Number and Post-Transductional Mechanisms Regulate TRAP1 Expression in Human Colorectal Carcinomas.

Authors - Michele Pietrafesa
  • KD Validated
View Article