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GSNOR,ADH5 Polyclonal antibody

GSNOR,ADH5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 11051-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存


经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue, mouse kidney tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman prostate cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11051-1-AP targets GSNOR,ADH5 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, chicken, zebrafish
免疫原 GSNOR,ADH5 fusion protein Ag1533 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alcohol dehydrogenase 5 (class III), chi polypeptide
别名 ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5
计算分子量 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC070491
基因名称 ADH5
Gene ID (NCBI) 128
RRIDAB_593422
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11766
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

实验方案

Product Specific Protocols
WB protocol for GSNOR,ADH5 antibody 11051-1-APDownload protocol
IHC protocol for GSNOR,ADH5 antibody 11051-1-APDownload protocol
IF protocol for GSNOR,ADH5 antibody 11051-1-APDownload protocol
IP protocol for GSNOR,ADH5 antibody 11051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Blood

Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency.

Authors - Anfeng Mu
  • KO Validated
humanWB

Nat Commun

Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells

Authors - Akiko Tomita
human,mouseWB,IHC

Mol Cell

Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans.

Authors - Felix A Dingler
  • KO Validated
mouseWB

Cancer Res

S-nitrosylation of the Mitochondrial Chaperone TRAP1 Sensitizes Hepatocellular Carcinoma Cells to Inhibitors of Succinate Dehydrogenase.

Authors - Salvatore Rizza
  • KO Validated
mouseWB

Antioxid Redox Signal

GSNOR deficiency enhances in situ skeletal muscle strength, fatigue resistance and RyR1 S-nitrosylation without impacting mitochondrial content and activity.

Authors - Younghye Moon
  • KO Validated
mouseWB

Redox Biol

GSNOR modulates hyperhomocysteinemia-induced T cell activation and atherosclerosis by switching Akt S-nitrosylation to phosphorylation.

Authors - Jing Li
  • KD Validated
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