验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, U2OS cells, HeLa cells |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human colon cancer tissue, human colon tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11644-2-AP targets Gelsolin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2239 Product name: Recombinant human Gelsolin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 433-782 aa of BC026033 Sequence: MAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQDEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIYKGGTSREGGQTAPASTRLFQVRANSAGATRAVEVLPKAGALNSNDAFVLKTPSAAYLWVGTGASEAEKTGAQELLRVLRAQPVQVAEGSEPDGFWEALGGKAAYRTSPRLKDKKMDAHPPRLFACSNKIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVKQGFEPPSFVGWFLGWDDDYWSVDPLDRAMAELAA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | gelsolin (amyloidosis, Finnish type) |
| 别名 | GSN, Actin-depolymerizing factor |
| 计算分子量 | 782 aa, 86 kDa |
| 观测分子量 | 87-93 kDa |
| GenBank蛋白编号 | BC026033 |
| 基因名称 | Gelsolin |
| Gene ID (NCBI) | 2934 |
| RRID | AB_2295090 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P06396 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease. Plasma gelsolin can interact with actin and form a complex of 130 kDa (PMID: 6330060, 3030756).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Gelsolin antibody 11644-2-AP | Download protocol |
| WB protocol for Gelsolin antibody 11644-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2. | ||
Mol Cell Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. | ||
J Clin Invest Loss of RPGR disrupts motile cilia and causes primary ciliary dyskinesia by affecting F-actin dynamics. | ||
Sci Adv Cytoskeletal remodeling promotes tunneling nanotube formation and drives cardiac resident cell mitochondrial transfer in sepsis. | ||
Adv Healthc Mater Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein. | ||
Cell Rep Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea. |
