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GSK3B Polyclonal antibody

GSK3B Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 24198-1-AP

Print datasheet

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta

验证数据展示

  • WB analysis of mouse brain using 24198-1-AP

    WB analysis of mouse brain using 24198-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 24198-1-AP

    IP experiment of mouse brain using 24198-1-AP

    IP result of anti-GSK3B (IP:24198-1-AP, 4ug; Detection:24198-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of human testis using 24198-1-AP

    IHC staining of human testis using 24198-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24198-1-AP (GSK3B Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with GSK3B antibody included
  • IHC staining of human testis using 24198-1-AP

    IHC staining of human testis using 24198-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24198-1-AP (GSK3B Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with GSK3B antibody included
  • IF Staining of HeLa using 24198-1-AP

    IF Staining of HeLa using 24198-1-AP

    Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 24198-1-AP (GSK3B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • FC experiment of HeLa using 24198-1-AP

    FC experiment of HeLa using 24198-1-AP

    1X10^6 HeLa cells were stained with .05ug GSK3B antibody (24198-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, PC-12 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inEthacrynic acid treated HeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GSK3B Ready-To-Use IHC Kit. GSK3B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.05 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

24198-1-AP targets GSK3B in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GSK3B fusion protein Ag21438 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycogen synthase kinase 3 beta
Synonyms GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta
Calculated Molecular Weight 433 aa, 48 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC000251
Gene Symbol GSK3B
Gene ID (NCBI) 2932
RRIDAB_2879451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP49841
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GSK3B antibody 24198-1-APDownload protocol
IHC protocol for GSK3B antibody 24198-1-APDownload protocol
IF protocol for GSK3B antibody 24198-1-APDownload protocol
IP protocol for GSK3B antibody 24198-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cardiovasc Med

Vascular Smooth Muscle FTO Promotes Aortic Dissecting Aneurysms via m6A Modification of Klf5.

Authors - Dong Ma
View Article
humanWB

Tumour Biol

Downregulation of β-catenin blocks fibrosis via Wnt2 signaling in human keloid fibroblasts.

Authors - Yumei Cai
View Article
humanWB

Medicine (Baltimore)

Wnt2 knock down by RNAi inhibits the proliferation of in vitro-cultured human keloid fibroblasts.

Authors - Yumei Cai
View Article

Biomed Res Int

Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling.

Authors - Xueyun Chen
View Article