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GSG1 Polyclonal antibody

GSG1 Polyclonal Antibody for IF-P, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, WB, ELISA

Conjugate

Unconjugated

Cat No : 15258-1-AP

Print datasheet

Synonyms

germ cell associated 1, GSG1

验证数据展示

  • WB analysis using 15258-1-AP

    WB analysis using 15258-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15258-1-AP (GSG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 15258-1-AP

    IF Staining of mouse testis using 15258-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using GSG1 antibody (15258-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IF detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15258-1-AP targets GSG1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, WB, ELISA
Tested Reactivity human, mouse
Immunogen GSG1 fusion protein Ag7200 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name germ cell associated 1
Synonyms germ cell associated 1, GSG1
Calculated Molecular Weight 37 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC001796
Gene Symbol GSG1
Gene ID (NCBI) 83445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2KHT4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSG1 causes the redistribution of PAPOLB from the cytosol to the endoplasmic reticulum. It has eight isoforms with MW 31-33 kDa and 36-40 kDa.

实验方案

Product Specific Protocols
WB protocol for GSG1 antibody 15258-1-APDownload protocol
IF protocol for GSG1 antibody 15258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols