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验证数据展示

  • WB analysis using 15258-1-AP

    WB analysis using 15258-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15258-1-AP (GSG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 15258-1-AP

    IF Staining of mouse testis using 15258-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using GSG1 antibody (15258-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

GSG1 Polyclonal antibody

GSG1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 15258-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

UNQ709/PRO1360, Germ cell-specific gene 1 protein, germ cell associated 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15258-1-AP targets GSG1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7200

Product name: Recombinant human GSG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-282 aa of BC001796

Sequence: MELSKAFSGQRTLLSAILSMLSLSFSTTSLLSNYWFVGTQKVPKPLCEKGLAAKCFDMPVSLDGDTNTSTQEVVQYNWETGDDRFSFRSFRSGMWLSCEETVEEPGERCRSFIELTPPAKRGEKGLLEFATLQGPCHPTLRFGGKRLMEKASLPSPPLGLCGKNPMVIPGNADHLHRTSIHQLPPATNRLATHWEPCLWAQTERLCCCFLCPVRSPGDGGPHDVFTSLPSDCQLGSRRLETTCLELWLGLLHGLALLHLLHGVGCHHLQHVHQDGAGVQVQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 germ cell associated 1
别名 UNQ709/PRO1360, Germ cell-specific gene 1 protein, germ cell associated 1
计算分子量 37 kDa
观测分子量39 kDa
GenBank蛋白编号BC001796
基因名称 GSG1
Gene ID (NCBI) 83445
RRIDAB_3085457
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2KHT4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSG1 causes the redistribution of PAPOLB from the cytosol to the endoplasmic reticulum. It has eight isoforms with MW 31-33 kDa and 36-40 kDa.

实验方案

Product Specific Protocols
IF protocol for GSG1 antibody 15258-1-APDownload protocol
WB protocol for GSG1 antibody 15258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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