• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

GRWD1 Polyclonal antibody

GRWD1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 10354-1-AP

Print datasheet

Synonyms

GRWD, GRWD1, KIAA1942, RRB1, WDR28

验证数据展示

  • WB analysis of HEK-293 using 10354-1-AP

    WB analysis of HEK-293 using 10354-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10354-1-AP

    IHC staining of human prostate cancer using 10354-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10354-1-AP (GRWD1 antibody) at dilution of 1:100 (under 25x lens).

  • IP experiment of PC-3 using 10354-1-AP

    IP experiment of PC-3 using 10354-1-AP

    IP result of anti-GRWD1 (IP:10354-1-AP, 4ug; Detection:10354-1-AP 1:1000) with PC-3 cells lysate 4000ug.

经过测试的应用

Positive WB detected inHEK-293 cells, PC-3 cells, SKOV-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10354-1-AP targets GRWD1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen GRWD1 fusion protein Ag0367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamate-rich WD repeat containing 1
Synonyms GRWD, GRWD1, KIAA1942, RRB1, WDR28
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC002440
Gene Symbol GRWD1
Gene ID (NCBI) 83743
RRIDAB_2114642
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQ67
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WD-repeat proteins are a class of functionally divergent molecules that cooperate with other proteins to regulate cellular processes. WD-repeat proteins have been implicated in a variety of cellular functions that are mostly regulatory in nature, including cytoskeleton organization, vesicular fusion, pre-mRNA splicing, and cell cycle regulation. WD-repeat-containing protein designated glutamate-rich WD repeat (GRWD1) is a component of the 50S and 80S preribosomal complexes and plays a role in ribosome biogenesis and during myeloid differentiation its levels are regulated. [PMID:15885502]

实验方案

Product Specific Protocols
WB protocol for GRWD1 antibody 10354-1-APDownload protocol
IHC protocol for GRWD1 antibody 10354-1-APDownload protocol
IP protocol for GRWD1 antibody 10354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Exp Cell Res

GRWD1 promotes cell proliferation and migration in non-small cell lung cancer by activating the Notch pathway.

Authors - Qiongzi Wang
View Article