验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GRP78/HSPA5 Ready-To-Use IHC Kit. GRP78/HSPA5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
80849-1-RR targets GRP78/BIP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | pig |
| 免疫原 |
CatNo: Ag2188 Product name: Recombinant human GRP78,BIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 351-654 aa of BC020235 Sequence: LKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
| 别名 | BIP, GRP78, HSPA5, 5F17, 78 kDa glucose-regulated protein |
| 计算分子量 | 78 kDa |
| 观测分子量 | 70,78 kDa |
| GenBank蛋白编号 | BC020235 |
| 基因名称 | GRP78/BIP |
| Gene ID (NCBI) | 3309 |
| RRID | AB_2918916 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11021 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GRP78/BIP antibody 80849-1-RR | Download protocol |
| IP protocol for GRP78/BIP antibody 80849-1-RR | Download protocol |
| WB protocol for GRP78/BIP antibody 80849-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
