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验证数据展示

  • WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

    WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • IP experiment of SGC-7901 using 66574-1-Ig (same clone as 66574-1-PBS)

    IP experiment of SGC-7901 using 66574-1-Ig (same clone as 66574-1-PBS)

    IP result of anti-GRP78/BIP (IP:66574-1-Ig, 5ug; Detection:66574-1-Ig 1:3000) with SGC-7901 cells lysate 2200 ug. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP78/HSPA5 antibody included
  • IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP78/HSPA5 antibody included
  • IF Staining of U2OS using 66574-1-Ig (same clone as 66574-1-PBS)

    IF Staining of U2OS using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed thapsigargin treated U2OS cells using GRP78/BIP antibody (66574-1-Ig, Clone: 1D6F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GRP78/BIP Monoclonal antibody, PBS Only

GRP78/BIP Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66574-1-PBS

产品说明书

CloneNo. 1D6F7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

BIP, GRP78, HSPA5, 1D6F7, 78 kDa glucose-regulated protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GRP78/HSPA5 Ready-To-Use IHC Kit. GRP78/HSPA5 primary antibody included.

产品信息

66574-1-PBS targets GRP78/BIP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2188

Product name: Recombinant human GRP78,BIP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 351-654 aa of BC020235

Sequence: LKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
别名 BIP, GRP78, HSPA5, 1D6F7, 78 kDa glucose-regulated protein
计算分子量 78 kDa
观测分子量 70-78 kDa
GenBank蛋白编号BC020235
基因名称 GRP78/BIP
Gene ID (NCBI) 3309
RRIDAB_2881934
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP11021
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

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