Recombinant human GRP78,BIP protein
Cat no : Ag2188
Synonyms
BIP, GRP78, GRP78/BIP, HSPA5, 78 kDa glucose-regulated protein
Validation Data Gallery View All
Product Information
| Peptide Sequence |
LKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL
(351-654 aa encoded by BC020235) |
| Activity | Not tested. |
| Endotoxin Level |
Please contact the lab for more information.
Note：Remove endotoxin and sterilization is required for cell assay. |
| Purity | 85%, by SDS-PAGE with Coomassie Brilliant Blue staining. |
| Formulation | The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.0). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 100mM GSH. |
Reconstitution and Storage
| Reconstitution |
Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
After reconstitution with sterile water, if glycerol has no effect on subsequent experiments, it is recommended to add an equal volume of glycerol for long-term storage (see Stability and Storage for more details). If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used). Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below). |
| Stability and Storage | Store for up to 12 months at -20°C to -80°C as lyophilized powder. |
| Storage of Reconstituted Protein |
Short-term storage: Store at 2-8°C for (1-2 weeks).
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles. |
Publications
| Species | Title |
|---|---|
Cell Rep circATF6 triggers calcium overload to synergize with EGFR-TKI in non-small cell lung cancer via modulation of proteostasis. | |
Free Radic Biol Med TRAF6 mediates vascular remodeling via endoplasmic reticulum stress-mitophagy in hypoxic pulmonary hypertension. |
