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验证数据展示

  • WB analysis using 86721-1-RR (same clone as 86721-1-PBS)

    WB analysis using 86721-1-RR (same clone as 86721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86721-1-RR (GRP75 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86721-1-PBS in a different storage buffer formulation.

  • WB analysis using 86721-1-RR (same clone as 86721-1-PBS)

    WB analysis using 86721-1-RR (same clone as 86721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86721-1-RR (GRP75 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86721-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86721-1-RR

    Affinity and Kinetic Characterization of 86721-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86721-1-RR against Human GRP75 were performed. The affinity constant is 1.66 nM.

GRP75 Recombinant monoclonal antibody, PBS Only

GRP75 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86721-1-PBS

产品说明书

CloneNo. 251670D1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

HSPA9, 75 kDa glucose-regulated protein, CSA, GRP-75, Heat shock 70 kDa protein 9

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HSPA9/GRP75 Ready-To-Use IHC Kit. HSPA9/GRP75 primary antibody included.

产品信息

86721-1-PBS targets GRP75 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag6673

Product name: Recombinant human GRP75 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 331-679 aa of BC000478

Sequence: YLTMDSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heat shock 70kDa protein 9 (mortalin)
别名 HSPA9, 75 kDa glucose-regulated protein, CSA, GRP-75, Heat shock 70 kDa protein 9
计算分子量 74 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC000478
基因名称 GRP75
Gene ID (NCBI) 3313
RRIDAB_3745077
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP38646
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. (PMID: 21640711, 22920904)

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