验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
产品信息
28482-1-AP targets GRP in WB, IHC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag29357 Product name: Recombinant human GRP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 49-120 aa of BC004488 Sequence: LMGKKSTGESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPKALGNQQPSWDSEDSSNFK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | gastrin-releasing peptide |
| 别名 | BN, gastrin releasing peptide, GRP, GRP 10, preproGRP, proGRP |
| 计算分子量 | 16 kDa |
| 观测分子量 | 16-18 kDa |
| GenBank蛋白编号 | BC004488 |
| 基因名称 | GRP |
| Gene ID (NCBI) | 2922 |
| RRID | AB_2881152 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07492 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GRP antibody 28482-1-AP | Download protocol |
| WB protocol for GRP antibody 28482-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ann N Y Acad Sci The von Economo neurons in the frontoinsular and anterior cingulate cortex. | ||
Front Immunol A novel necroptosis-related gene index for predicting prognosis and a cold tumor immune microenvironment in stomach adenocarcinoma |
