mGluR1 Polyclonal antibody
mGluR1 Polyclonal Antibody for FC, IF/ICC, IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC, IF/ICC, IHC,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue |
| Positive FC detected in | SH-SY5Y cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19955-1-AP targets mGluR1 in IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | FC, IF/ICC, IHC,ELISA |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | glutamate receptor, metabotropic 1 |
| Synonyms | GPRC1A, GRM1, GRM1A, mGlu1, mGluR1, MGLUR1A |
| Calculated Molecular Weight | 132 kDa |
| GenBank Accession Number | NM_000838 |
| Gene Symbol | GRM1 |
| Gene ID (NCBI) | 2911 |
| RRID | AB_10638312 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13255 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GRM1, also named as GPRC1A and MGLUR1, belongs to the G-protein coupled receptor 3 family. GRM1 is a receptor for glutamate. The activity of GRM1 is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. It may participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. The antibody can recognize isoform1 and isoform2 of GRM1.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for mGluR1 antibody 19955-1-AP | Download protocol |
| IF protocol for mGluR1 antibody 19955-1-AP | Download protocol |
| FC protocol for mGluR1 antibody 19955-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Cancer Res Serum glutamate levels correlate with Gleason score and glutamate blockade decreases proliferation, migration, and invasion and induces apoptosis in prostate cancer cells. | ||
Int J Biol Sci Glutamate signaling in benign and malignant disorders: current status, future perspectives, and therapeutic implications. | ||
Brain Commun M1 muscarinic acetylcholine receptor dysfunction in moderate Alzheimer's disease pathology. |
