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验证数据展示

  • WB analysis using 21920-1-AP

    WB analysis using 21920-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 21920-1-AP

    IP experiment of mouse brain using 21920-1-AP

    IP result of anti-NMDAR2B/GRIN2B (IP:21920-1-AP, 3ug; Detection:21920-1-AP 1:2000) with mouse brain tissue lysate 6000ug.

  • IHC staining of mouse brain using 21920-1-AP

    IHC staining of mouse brain using 21920-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 21920-1-AP

    IHC staining of mouse brain using 21920-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 21920-1-AP

    IF Staining of mouse brain using 21920-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR2B/GRIN2B antibody (21920-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of SH-SY5Y using 21920-1-AP

    FC experiment of SH-SY5Y using 21920-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug NMDAR2B/GRIN2B antibody (21920-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • IF Staining of mouse brain using 21920-1-AP

    IF Staining of mouse brain using 21920-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NMDAR2B/GRIN2B antibody (21920-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

NMDAR2B/GRIN2B Polyclonal antibody

NMDAR2B/GRIN2B Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA
Cat No. 21920-1-AP

产品说明书

引用文献(116)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA and More (2)

别名

GRIN2B, NMDAR2B, NR2B, GluN2B, Glutamate receptor ionotropic, NMDA 2B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF-Fro detected inmouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21920-1-AP targets NMDAR2B/GRIN2B in WB, IHC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, zebra finch
免疫原

CatNo: Ag16318

Product name: Recombinant human GRIN2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1133-1485 aa of BC113620

Sequence: YLDQFRTKENSPHWEHVDLTDIYKERSDDFKRDSVSGGGPCTNRSHIKHGTGDKHGVVSGVPAPWEKNLTNVEWEDRSGGNFCRSCPSKLHNYSTTVTGQNSGRQACIRCEACKKAGNLYDISEDNSLQELDQPAAPVAVTSNASTTKYPQSPTNSKAQKKNRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRFMDGSPYAHMFEMSAGESTFANNKSSVPTAGHHHHNNPGGGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHGSKSYFFRQPTVAGASKARPDFRALVTNKPVVSALHGAVPARFQKDICIGNQSNPCVPNNKNPRAFNGSSNGHVYEKLSSIESDV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutamate receptor, ionotropic, N-methyl D-aspartate 2B
别名 GRIN2B, NMDAR2B, NR2B, GluN2B, Glutamate receptor ionotropic, NMDA 2B
计算分子量 1484 aa, 166 kDa
观测分子量 166 kDa
GenBank蛋白编号BC113620
基因名称 GRIN2B
Gene ID (NCBI) 2904
RRIDAB_11232223
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13224
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRIN2B (also known as GluN2B or NMDAR2B) is a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. NMDA receptors are widely expressed in the central nervous system and play a major role in excitatory synaptic transmission and plasticity (PMID: 23223336). NMDA receptors large multi-subunit complexes arranged into heteromeric assemblies composed of four homologous subunits within a repertoire of over 10 different subunits: eight GluN1 isoforms, four GluN2 subunits (A-D) and two GluN3 subunits (A and B) (PMID: 21395862). Naturally occurring mutations within GRIN2B gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia.

实验方案

Product Specific Protocols
IF protocol for NMDAR2B/GRIN2B antibody 21920-1-APDownload protocol
IHC protocol for NMDAR2B/GRIN2B antibody 21920-1-APDownload protocol
IP protocol for NMDAR2B/GRIN2B antibody 21920-1-APDownload protocol
WB protocol for NMDAR2B/GRIN2B antibody 21920-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB

Sci Bull (Beijing)

Tumor cell-derived N-acetyl-aspartyl-glutamate reshapes the tumor microenvironment to facilitate breast cancer metastasis

Authors - Jie Xia
  • KD Validated
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mouseWB

Adv Sci (Weinh)

Single-Nucleus RNA Sequencing Reveals that Decorin Expression in the Amygdala Regulates Perineuronal Nets Expression and Fear Conditioning Response after Traumatic Brain Injury.

Authors - Yingwu Shi
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mouseWB

Sci Adv

Loss of schizophrenia-related miR-501-3p in mice impairs sociability and memory by enhancing mGluR5-mediated glutamatergic transmission

Authors - Wenquan Liang
View Article
mouseWB

Sci Adv

RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission

Authors - Pengcheng Ma
View Article
humanWB,CoIP

J Clin Invest

TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.

Authors - Haiqing Zhang
View Article
mouseWB

Mol Neurodegener

Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.

Authors - Qingyang Zhang
View Article
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