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GRIN1 Monoclonal antibody

GRIN1 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Chicken and More (1)

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H2C2

Cat No : 67717-1-Ig

Print datasheet

Synonyms

GRIN1, NR1

验证数据展示

  • WB analysis using 67717-1-Ig

    WB analysis using 67717-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67717-1-Ig (GRIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 67717-1-Ig

    IHC staining of mouse cerebellum using 67717-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67717-1-Ig (GRIN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of MCF-7 using 67717-1-Ig

    FC experiment of MCF-7 using 67717-1-Ig

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human GRIN1 (67717-1-Ig, Clone:1H2C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inpig brain tissue, rabbit cerebellum tissue, rat brain tissue, mouse brain tissue, chicken brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67717-1-Ig targets GRIN1 in WB, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Chicken samples.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat, Rabbit, Chicken
Cited Reactivitymouse
Immunogen GRIN1 fusion protein Ag26364 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 1
Synonyms GRIN1, NR1
Calculated Molecular Weight 105 kDa
Observed Molecular Weight105120 kDa
GenBank Accession NumberNM_000832
Gene Symbol GRIN1
Gene ID (NCBI) 2902
RRIDAB_2882906
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRIN1 antibody 67717-1-IgDownload protocol
IHC protocol for GRIN1 antibody 67717-1-IgDownload protocol
FC protocol for GRIN1 antibody 67717-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Exp Brain Res

The NR2B-targeted intervention alleviates the neuronal injuries at the sub-acute stage of cerebral ischemia: an exploration of stage-dependent strategy against ischemic insults

Authors - Bei Guo
View Article