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GRIK2 Polyclonal antibody

GRIK2 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13597-1-AP

Print datasheet

Synonyms

EAA4, GLR6, GluK2, GluR 6, GLUR6, Glutamate receptor 6, GRIK2, MRT6

验证数据展示

  • WB analysis of human brain using 13597-1-AP

    WB analysis of human brain using 13597-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13597-1-AP (GRIK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 13597-1-AP

    FC experiment of SH-SY5Y using 13597-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug GRIK2 antibody (13597-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inhuman brain tissue
Positive FC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

13597-1-AP targets GRIK2 in WB, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, WB,ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GRIK2 fusion protein Ag4516 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamate receptor, ionotropic, kainate 2
Synonyms EAA4, GLR6, GluK2, GluR 6, GLUR6, Glutamate receptor 6, GRIK2, MRT6
Calculated Molecular Weight 908 aa, 103 kDa
Observed Molecular Weight 93-120 kDa
GenBank Accession NumberBC037954
Gene Symbol GRIK2
Gene ID (NCBI) 2898
RRIDAB_10640301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13002
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRIK2 antibody 13597-1-APDownload protocol
FC protocol for GRIK2 antibody 13597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Mol Med

Differentiation of adult human retinal pigment epithelial cells into dopaminergic-like cells in vitro and in the recipient monkey brain.

Authors - Sha Li
View Article