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GRIK1 Polyclonal antibody

GRIK1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25779-1-AP

Print datasheet

Synonyms

EAA3, EEA3, GLR5, GluK1, GluR 5, GLUR5, Glutamate receptor 5, GRIK1

验证数据展示

  • WB analysis using 25779-1-AP

    WB analysis using 25779-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25779-1-AP (GRIK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 25779-1-AP

    IHC staining of human cerebellum using 25779-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25779-1-AP (GRIK1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human cerebellum using 25779-1-AP

    IHC staining of human cerebellum using 25779-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25779-1-AP (GRIK1 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25779-1-AP is GRIK1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GRIK1 fusion protein Ag22664 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamate receptor, ionotropic, kainate 1
Synonyms EAA3, EEA3, GLR5, GluK1, GluR 5, GLUR5, Glutamate receptor 5, GRIK1
Calculated Molecular Weight 918 aa, 104 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC156975
Gene Symbol GRIK1
Gene ID (NCBI) 2897
RRIDAB_2880236
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP39086
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRIK1 antibody 25779-1-APDownload protocol
IHC protocol for GRIK1 antibody 25779-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Exp Ther Med

Glutamate receptor ionotropic, kainate 1 serves as a novel tumor suppressor of colorectal carcinoma and predicts clinical prognosis.

Authors - Zhong Ren
View Article