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验证数据展示

  • WB analysis using 21727-1-AP

    WB analysis using 21727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21727-1-AP (GRID2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 21727-1-AP

    WB analysis using 21727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21727-1-AP (GRID2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 21727-1-AP

    IHC staining of mouse cerebellum using 21727-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21727-1-AP (GRID2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 21727-1-AP

    IHC staining of mouse cerebellum using 21727-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21727-1-AP (GRID2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GRID2 Polyclonal antibody

GRID2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21727-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GluR delta 2 subunit, GLURD2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21727-1-AP targets GRID2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16391

Product name: Recombinant human GRID2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-358 aa of BC099652

Sequence: SANADSIIHIGAIFDESAKKDDEVFRTAVGDLNQNEEILQTEKITFSVTFVDGNNPFQAVQEACELMNQGILALVSSIGCTSAGSLQSLADAMHIPHLFIQRSTAGTPRSGCGLTRSNRNDDYTLSVRPPVYLHDVILRVVTEYAWQKFIIFYDSEYDIRGIQEFLDKVSQQGMDVALQKVENNINKMITTLFDTMRIEELNRYRDTLRRAILVMNPATAKSFITEVVETNLVAFDCHWIIINEEINDVDVQELVRRSIGRLTIIRQTFPVPQNISQRCFRGNHRISSTLCDPKDPFAQNMEISNLYIYDTVLLLANAFHKKLEDRKWHSMASLSCIRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutamate receptor, ionotropic, delta 2
别名 GluR delta 2 subunit, GLURD2
计算分子量 1007 aa, 113 kDa
观测分子量113 kDa
GenBank蛋白编号BC099652
基因名称 GRID2
Gene ID (NCBI) 2895
RRIDAB_3742053
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43424
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The GRID2 gene provides instructions for making a protein called the glutamate receptor ionotropic, delta-2 subunit (GluD2) . While it belongs to the family of ionotropic glutamate receptors, which are proteins that respond to the neurotransmitter glutamate by opening an ion channel, GluD2 is unique. It is often referred to as an "orphan" receptor because it does not bind glutamate or function as a typical ion channel. Instead, its primary roles are structural and organizational, critical for the development, maintenance, and function of specific synapses in the brain.

实验方案

Product Specific Protocols
IHC protocol for GRID2 antibody 21727-1-APDownload protocol
WB protocol for GRID2 antibody 21727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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