验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, mouse brain tissue, mouse cerebellum tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23350-1-AP targets GRIA4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag19949 Product name: Recombinant human GRIA4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 87-229 aa of BC167786 Sequence: YSRGVFAIFGLYDKRSVHTLTSFCSALHISLITPSFPTEGESQFVLQLRPSLRGALLSLLDHYEWNCFVFLYDTDRGYSILQAIMEKAGQNGWHVSAICVENFNDVSYRQLLEELDRRQEKKFVIDCEIERLQNILEQIVSVG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutamate receptor, ionotrophic, AMPA 4 |
| 别名 | GluA4, GluR 4, GluR D, GLUR4, GLUR4C, GLURD, Glutamate receptor 4, GRIA4 |
| 计算分子量 | 902 aa, 101 kDa |
| 观测分子量 | 101 kDa |
| GenBank蛋白编号 | BC167786 |
| 基因名称 | GRIA4 |
| Gene ID (NCBI) | 2893 |
| RRID | AB_2879262 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P48058 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for GRIA4 antibody 23350-1-AP | Download protocol |
| IHC protocol for GRIA4 antibody 23350-1-AP | Download protocol |
| IP protocol for GRIA4 antibody 23350-1-AP | Download protocol |
| WB protocol for GRIA4 antibody 23350-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Psychiatry A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling. | ||
J Clin Invest TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation. | ||
Front Cell Dev Biol Olfactomedin-3 Enhances Seizure Activity by Interacting With AMPA Receptors in Epilepsy Models. | ||
Mol Brain Assessments of prolonged effects of desflurane and sevoflurane on motor learning deficits in aged AppNL-G-F/NL-G-F mice. |
