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验证数据展示

  • WB analysis using 28699-1-AP (same clone as 28699-1-PBS)

    WB analysis using 28699-1-AP (same clone as 28699-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28699-1-AP (GREB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 28699-1-AP (same clone as 28699-1-PBS)

    IHC staining of human liver using 28699-1-AP (same clone as 28699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28699-1-AP (GREB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 28699-1-AP (same clone as 28699-1-PBS)

    IF Staining of HepG2 using 28699-1-AP (same clone as 28699-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GREB1 antibody (28699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28699-1-PBS in a different storage buffer formulation.

GREB1 Polyclonal antibody, PBS Only

GREB1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28699-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28699-1-PBS targets GREB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29908

Product name: Recombinant human GREB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1075-1212 aa of NM_014668

Sequence: EVPLEKGARNEALESDAEKLSSTDNEDEELGTEGSTSEKRSPMKRERSRSHDSASSSLSSKASGSALGGESSAQPTALPQGEHARSPQPRGPAEEGRAPGEKQRPRASQGPPSAISRHSPGPTPQPDCSLRTGQRSVQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GREB1 protein
别名
计算分子量 216 kDa
观测分子量 216-240 kDa
GenBank蛋白编号NM_014668
基因名称 GREB1
Gene ID (NCBI) 9687
RRIDAB_2881196
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4ZG55
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GREB1 (Gene regulated by estrogen in breast cancer protein1) encodes a 1949-amino acid protein that contains an N-terminal Zn-binding domain followed by a circularly permuted catalytically inactive superfamily II helicase domain and a C-terminal Fringe-like GT domain. GREB1 is an early estrogen-responsive gene, and its expression is correlated with estrogen levels in breast cancer patients. GREB1 is implicated in hormone-dependent cancers, such as ovarian, prostate cancer and as a risk factor in endometriosis and variations in bone mineral density.

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