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验证数据展示

  • WB analysis of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

    WB analysis of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

  • IP experiment of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

    IP experiment of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

    IP result of anti-GRB7 (IP:10045-1-Ig, 3ug; Detection:10045-1-Ig 1:500) with A431 cells lysate 3300ug. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 10045-1-Ig (same clone as 10045-1-PBS)

    WB analysis of HEK-293 using 10045-1-Ig (same clone as 10045-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10045-1-Ig (same clone as 10045-1-PBS)

    IHC staining of human colon cancer using 10045-1-Ig (same clone as 10045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10045-1-Ig (GRB7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRB7 antibody included
  • IHC staining of human colon cancer using 10045-1-Ig (same clone as 10045-1-PBS)

    IHC staining of human colon cancer using 10045-1-Ig (same clone as 10045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10045-1-Ig (GRB7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRB7 antibody included
  • IF Staining of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

    IF Staining of A431 using 10045-1-Ig (same clone as 10045-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GRB7 antibody (10045-1-Ig) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GRB7 Polyclonal antibody, PBS Only

GRB7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10045-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GRB7 Ready-To-Use IHC Kit. GRB7 primary antibody included.

产品信息

10045-1-PBS targets GRB7 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0023

Product name: Recombinant human GRB7 protein

Source: e coli.-derived, Unknow

Tag: N/A

Domain: Gst-KG-Grb7 of BC006535

Sequence:

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth factor receptor-bound protein 7
别名
计算分子量 532 aa, 60 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC006535
基因名称 GRB7
Gene ID (NCBI) 2886
RRIDAB_2232601
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14451
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Grb7 belongs to a family of adapter proteins. This family, including Grb10, Grb7 and Grb 14, contains a praline-rich, a RA, a PH, a BPS and SH2 domain. Grb7 associate with a number of receptors including EGFR and FAK. Functions of Grb7 include mobility because it associates receptors such FAK, and direct evidence from overexpression of this proteins.This antibody recognize the N-terminal of GRB7.

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