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验证数据展示

  • WB analysis using 66880-1-Ig (same clone as 66880-1-PBS)

    WB analysis using 66880-1-Ig (same clone as 66880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66880-1-Ig (GRB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66880-1-Ig (same clone as 66880-1-PBS)

    WB analysis of HEK-293 using 66880-1-Ig (same clone as 66880-1-PBS)

    WB result of GRB2 antibody (66880-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRB2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

  • WB analysis using 66880-1-Ig (same clone as 66880-1-PBS)

    WB analysis using 66880-1-Ig (same clone as 66880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66880-1-Ig (GRB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66880-1-Ig (same clone as 66880-1-PBS)

    IHC staining of human pancreas cancer using 66880-1-Ig (same clone as 66880-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66880-1-Ig (GRB2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRB2 antibody included
  • IHC staining of human pancreas cancer using 66880-1-Ig (same clone as 66880-1-PBS)

    IHC staining of human pancreas cancer using 66880-1-Ig (same clone as 66880-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66880-1-Ig (GRB2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66880-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRB2 antibody included
  • Featured Product
  • KD/KO验证

GRB2 Monoclonal antibody, PBS Only

GRB2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66880-1-PBS

产品说明书

CloneNo. 1C5D11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

1C5D11, ASH, EGFRBP GRB2, Grb33, Growth factor receptor-bound protein 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GRB2 Ready-To-Use IHC Kit. GRB2 primary antibody included.

产品信息

66880-1-PBS targets GRB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0395

Product name: Recombinant human GRB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-214 aa of BC000631

Sequence: MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 growth factor receptor-bound protein 2
别名 1C5D11, ASH, EGFRBP GRB2, Grb33, Growth factor receptor-bound protein 2
计算分子量 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC000631
基因名称 GRB2
Gene ID (NCBI) 2885
RRIDAB_2882212
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62993
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-β protein precursor (AβPP). Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.