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验证数据展示

  • WB analysis of HepG2 using 16622-1-AP

    WB analysis of HepG2 using 16622-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16622-1-AP (GRB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16622-1-AP

    IF Staining of HepG2 using 16622-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16622-1-AP (GRB10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GRB10 Polyclonal antibody

GRB10 Polyclonal Antibody for WB, IF, ELISA
Cat No. 16622-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16622-1-AP targets GRB10 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9921

Product name: Recombinant human GRB10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 208-536 aa of BC024285

Sequence: MVTWCQQSNGSQTQLLQNFLNSSSCPEIQGFLHVKELGKKSWKKLYVCLRRSGLYCSTKGTSKEPRHLQLLADLEDSNIFSLIAGRKQYNAPTDHGLCIKPNKVRNETKELRLLCAEDEQTRTCWMTAFRLLKYGMLLYQNYRIPQQRKALLSPFSTPVRSVSENSLVAMDFSGQTGRVIENPAEAQSAALEEGHAWRKRSTRMNILGSQSPLHPSTLSTVIHRTQHWFHGRISREESHRIIKQQGLVDGLFLLRDSQSNPKAFVLTLCHHQKIKNFQILPCEDDGQTFFSLDDGNTKFSDLIQLVDFYQLNKGVLPCKLKHHCIRVAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth factor receptor-bound protein 10
别名
计算分子量536aa,61 kDa; 594aa,67 kDa
观测分子量 65 kDa
GenBank蛋白编号BC024285
基因名称 GRB10
Gene ID (NCBI) 2887
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13322
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Growth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the ins (INSR) and ins-like growth factor (IGF1R) receptors. It may play a role in mediating ins-stimulated ubiquitination of INSR, leading to proteasomal degradation.

实验方案

Product Specific Protocols
WB protocol for GRB10 antibody 16622-1-APDownload protocol
IF protocol for GRB10 antibody 16622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols