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验证数据展示

  • WB analysis of HepG2 using 16622-1-AP

    WB analysis of HepG2 using 16622-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16622-1-AP (GRB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16622-1-AP

    IF Staining of HepG2 using 16622-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16622-1-AP (GRB10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GRB10 Polyclonal antibody

GRB10 Polyclonal Antibody for WB, IF, ELISA
Cat No. 16622-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16622-1-AP targets GRB10 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GRB10 fusion protein Ag9921 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth factor receptor-bound protein 10
别名
计算分子量536aa,61 kDa; 594aa,67 kDa
观测分子量 65 kDa
GenBank蛋白编号BC024285
基因名称 GRB10
Gene ID (NCBI) 2887
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13322
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Growth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the ins (INSR) and ins-like growth factor (IGF1R) receptors. It may play a role in mediating ins-stimulated ubiquitination of INSR, leading to proteasomal degradation.

实验方案

Product Specific Protocols
WB protocol for GRB10 antibody 16622-1-APDownload protocol
IF protocol for GRB10 antibody 16622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols