验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
| IF | See 2 publications below |
产品信息
10747-2-AP targets GRASP65,GORASP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GRASP65,GORASP1 fusion protein Ag1054 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | golgi reassembly stacking protein 1, 65kDa |
| 别名 | GORASP1, GRASP65, GOLPH5, Golgi reassembly-stacking protein of 65 kDa, Golgi reassembly-stacking protein 1 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC008928 |
| 基因名称 | GRASP65 |
| Gene ID (NCBI) | 64689 |
| RRID | AB_2113204 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BQQ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. (refer to Wikipedia)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GRASP65,GORASP1 antibody 10747-2-AP | Download protocol |
| IF protocol for GRASP65,GORASP1 antibody 10747-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Cytosolic Ca2+ Modulates Golgi Structure Through PKCα-Mediated GRASP55 Phosphorylation. | ||
J Cell Biochem Golgi apparatus fragmentation participates in oxidized low-density lipoprotein-induced endothelial cell injury. | ||
Biochem Biophys Res Commun Golgi damage caused by dysfunction of PiT-2 in primary familial brain calcification | ||
Cell Death Dis GOLPH3 promotes endotoxemia-induced liver and kidney injury through Golgi stress-mediated apoptosis and inflammatory response | ||
Prostaglandins Other Lipid Mediat GOLPH3 knockdown alleviates the inflammation and apoptosis in lipopolysaccharide-induced acute lung injury by inhibiting Golgi stress mediated autophagy |