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GRAMD1B Polyclonal antibody

GRAMD1B Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24905-1-AP

Print datasheet

Synonyms

GRAM domain containing 1B, GRAMD1B, KIAA1201

验证数据展示

  • FC experiment of A549 using 24905-1-AP

    FC experiment of A549 using 24905-1-AP

    1X10^6 A549 cells were intracellularly stained with 0.5 ug Anti-Human GRAMD1B (24905-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of A549 using 24905-1-AP

    WB analysis of A549 using 24905-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24905-1-AP (GRAMD1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 24905-1-AP

    IHC staining of human liver cancer using 24905-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24905-1-AP (GRAMD1B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 24905-1-AP

    IHC staining of human liver cancer using 24905-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24905-1-AP (GRAMD1B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 24905-1-AP

    IF Staining of A549 using 24905-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 24905-1-AP (GRAMD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Positive FC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24905-1-AP targets GRAMD1B in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen GRAMD1B fusion protein Ag20670 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GRAM domain containing 1B
Synonyms GRAM domain containing 1B, GRAMD1B, KIAA1201
Calculated Molecular Weight 738 aa, 85 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC107480
Gene Symbol GRAMD1B
Gene ID (NCBI) 57476
RRIDAB_2879791
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3KR37
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRAMD1B, also named as KIAA1201, contains a transmembrane region and two domains of known function; the GRAM domain and a VASt domain. It is predicted to localize in the nucleus, supported by several nuclear transport signals and nuclearly associated motifs. This highly conserved gene is found in a variety of vertebrates and invertebrates, however, it is not found in bacteria or fungi. There're some isoforms with MW 50 kDa and 81-87 kDa. With phosphor modification, it's about 90-110 kDa in WB detection.

实验方案

Product Specific Protocols
WB protocol for GRAMD1B antibody 24905-1-APDownload protocol
IHC protocol for GRAMD1B antibody 24905-1-APDownload protocol
IF protocol for GRAMD1B antibody 24905-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Metab

Aster-B coordinates with Arf1 to regulate mitochondrial cholesterol transport.

Authors - John-Paul Andersen
View Article
humanWB,IF

J Lipid Res

ASTER-B regulates mitochondrial carotenoid transport and homeostasis

Authors - Sepalika Bandara
  • KD Validated
View Article
humanWB

Noncoding RNA

MiR-4646-5p Acts as a Tumor-Suppressive Factor in Triple Negative Breast Cancer and Targets the Cholesterol Transport Protein GRAMD1B

Authors - Katharina Jonas
View Article