验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, human placenta tissue, human brain tissue, Transfected HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13501-1-AP targets GPX7 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, chicken |
| 免疫原 |
CatNo: Ag4328 Product name: Recombinant human GPX7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 58-187 aa of BC032788 Sequence: GFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDSNKEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYLAQTSGKEPTWNFWKYLVAPDGKVVGAWDPTVSVEEVRPQITALVRKLILLKREDL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutathione peroxidase 7 |
| 别名 | CL683, EC:1.11.1.9, GPx 7, GPX6, GPx-7 |
| 计算分子量 | 187 aa, 21 kDa |
| 观测分子量 | 18-21 kDa |
| GenBank蛋白编号 | BC032788 |
| 基因名称 | GPX7 |
| Gene ID (NCBI) | 2882 |
| RRID | AB_2112560 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96SL4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPX7(Glutathione peroxidase 7) is also named as GPX6,CL683,GSHPx-7 and belongs to the glutathione peroxidase family.It shares approximately 45% amino acid sequence with other GPX family members.Because GPX7 expression protected cells from acidic bile acidinduced DNA damage, it may protect oesophageal epithelial cells from acidic bile acid-induced apoptosis(PMID:22157330). This antibody can ve detected as 18-21kDa due to signal peptide splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPX7 antibody 13501-1-AP | Download protocol |
| WB protocol for GPX7 antibody 13501-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gut Glutathione peroxidase 7 has potential tumour suppressor functions that are silenced by location-specific methylation in oesophageal adenocarcinoma. | ||
EMBO Mol Med Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human. | ||
Oncogene Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling. | ||
Carcinogenesis Loss of glutathione peroxidase 7 promotes TNF-α-induced NF-κB activation in Barrett's carcinogenesi.
| ||
Free Radic Biol Med GPx8 peroxidase prevents leakage of H2O2 from the endoplasmic reticulum. |
