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GPX4 (Human Specific) Recombinant antibody

GPX4 (Human Specific) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

6E9

Cat No : 82822-2-RR

Print datasheet

Synonyms

Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx

验证数据展示

  • WB analysis using 82822-2-RR

    WB analysis using 82822-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82822-2-RR (GPX4 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:15000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82822-2-RR targets GPX4 (Human Specific) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen GPX4 (Human Specific) fusion protein Ag30650 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Synonyms Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC021567
Gene Symbol GPX4
Gene ID (NCBI) 2879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36969
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701).82822-2-RR is human specific.

实验方案

Product Specific Protocols
WB protocol for GPX4 (Human Specific) antibody 82822-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols