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GPX4 Monoclonal antibody

GPX4 Monoclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig, dog, hamster, chicken, zebrafish and More (5)

Applications

WB, IF/ICC, IHC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

3F5G5

Cat No : 67763-1-Ig

Print datasheet

Synonyms

Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx



经过测试的应用

Positive WB detected inEC109 cells, NCCIT cells, rabbit testis tissue, pig brain tissue, human platelet tissue, rat testis tisssue, mouse testis tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue, Hela cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, 4T1 cells, ATDC-5 cells, MDCK cells, CHO cells, Chicken brain tissue, Zebrafish tissue
Positive IHC detected inhuman liver cancer tissue, mouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67763-1-Ig targets GPX4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig, dog, hamster, chicken, zebrafish samples.

Tested Applications WB, IF/ICC, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IP, CoIP, IF
Tested Reactivity Human, mouse, rat, rabbit, pig, dog, hamster, chicken, zebrafish
Cited Reactivityhuman, mouse, rat, pig, chicken, bovine, goat, sheep, duck
Immunogen GPX4 fusion protein Ag30650 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Synonyms Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC021567
Gene Symbol GPX4
Gene ID (NCBI) 2879
RRIDAB_2909469
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36969
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

实验方案

Product Specific Protocols
WB protocol for GPX4 antibody 67763-1-IgDownload protocol
IHC protocol for GPX4 antibody 67763-1-IgDownload protocol
IF protocol for GPX4 antibody 67763-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Host Microbe

Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice

Authors - Yunong Zeng
human,mouseWB

Nucleic Acids Res

MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation

Authors - Bangming Jin
mouseWB

Redox Biol

A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease

Authors - Yi Sun
mouseWB

Drug Des Devel Ther

Vancomycin Induced Ferroptosis in Renal Injury Through the Inactivation of Recombinant Glutathione Peroxidase 4 and the Accumulation of Peroxides

Authors - Xuedong Yin
humanWB

J Adv Res

Dehydrocostus lactone suppresses gastric cancer progression by targeting ACLY to inhibit fatty acid synthesis and autophagic flux

Authors - Yuxuan Chen
ratWB

Redox Biol

SSBP1 drives high fructose-induced glomerular podocyte ferroptosis via activating DNA-PK/p53 pathway.

Authors - Wen-Yuan Wu