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验证数据展示

  • WB analysis using 83592-4-RR (same clone as 83592-4-PBS)

    WB analysis using 83592-4-RR (same clone as 83592-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83592-4-RR (GPSN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83592-4-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 83592-4-RR (same clone as 83592-4-PBS)

    IF Staining of U-251 using 83592-4-RR (same clone as 83592-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using GPSN2 antibody (83592-4-RR, Clone: 240472D4 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 83592-4-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83592-4-RR (same clone as 83592-4-PBS)

    FC experiment of U-251 using 83592-4-RR (same clone as 83592-4-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug GPSN2 Recombinant antibody (83592-4-RR, Clone:240472D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83592-4-PBS in a different storage buffer formulation.

GPSN2 Recombinant monoclonal antibody, PBS Only

GPSN2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83592-4-PBS

产品说明书

CloneNo. 240472D4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

240472D4, EC:1.3.1.93, SC2, Synaptic glycoprotein SC2, TECR

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83592-4-PBS targets GPSN2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32598

Product name: Recombinant human GPSN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-87 aa of BC002425

Sequence: MKHYEVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPVGTTATLYFRDLGAQISW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glycoprotein, synaptic 2
别名 240472D4, EC:1.3.1.93, SC2, Synaptic glycoprotein SC2, TECR
计算分子量 36 kDa
观测分子量32-36 kDa
GenBank蛋白编号BC002425
基因名称 GPSN2
Gene ID (NCBI) 9524
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NZ01
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycoprotein synaptic 2 (GPSN2), also known as TER and TECR, functions as a trans-2,3-enoyl-CoA reductase and reduces long and very long chain fatty acid trans-2,3-enoyl-CoA substrates (PMID: 12482854).

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