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GPSM2 Polyclonal antibody

GPSM2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26798-1-AP

Print datasheet

Synonyms

GPSM2, LGN, Mosaic protein LGN, Pins

验证数据展示

  • WB analysis using 26798-1-AP

    WB analysis using 26798-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 26798-1-AP

    IHC staining of human placenta using 26798-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 26798-1-AP

    IHC staining of human placenta using 26798-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 26798-1-AP

    IF Staining of MCF-7 using 26798-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 26798-1-AP (GPSM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, HEK-293T cells, mouse liver tissue, HEK-293 cells
Positive IHC detected inhuman placenta tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26798-1-AP targets GPSM2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen GPSM2 fusion protein Ag25195 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G-protein signaling modulator 2 (AGS3-like, C. elegans)
Synonyms GPSM2, LGN, Mosaic protein LGN, Pins
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 70-77 kDa
GenBank Accession NumberBC027732
Gene Symbol GPSM2
Gene ID (NCBI) 29899
RRIDAB_2880640
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP81274
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPSM2 belongs to a family of proteins that modulate activation of G proteins. GPSM2 assists in the exchange of guanine nucleotides, and allows extracellular signals to be transmitted to cells via cell surface, and ultimately plays a key role in the activation of G-proteins. Therefore, GPSM2 is a critical factor for the stability of cell division. Some recent studies have shown that GPSM2 messenger RNA (mRNA) is overexpressed and plays a positive role in the development of certain tumors, such as liver cancer, pancreatic cancer, breast cancer. It also plays a role in neuroblast division and in the development of normal hearing. Mutations in GPSM2 are associated with autosomal recessive nonsyndromic deafness (DFNB82), which is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss.

实验方案

Product Specific Protocols
WB protocol for GPSM2 antibody 26798-1-APDownload protocol
IHC protocol for GPSM2 antibody 26798-1-APDownload protocol
IF protocol for GPSM2 antibody 26798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols