验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse heart tissue, HEK-293T cells, mouse liver tissue, HEK-293 cells |
| Positive IHC detected in | human placenta tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
26798-1-AP targets GPSM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag25195 Product name: Recombinant human GPSM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 559-600 aa of BC027732 Sequence: FSNLPGLRLTQNSQSVLSHLMTNDNKEADEDFFDILVKCQGS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G-protein signaling modulator 2 (AGS3-like, C. elegans) |
| 别名 | GPSM2, LGN, Mosaic protein LGN, Pins |
| 计算分子量 | 75 kDa |
| 观测分子量 | 70-77 kDa |
| GenBank蛋白编号 | BC027732 |
| 基因名称 | GPSM2 |
| Gene ID (NCBI) | 29899 |
| RRID | AB_2880640 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P81274 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPSM2 belongs to a family of proteins that modulate activation of G proteins. GPSM2 assists in the exchange of guanine nucleotides, and allows extracellular signals to be transmitted to cells via cell surface, and ultimately plays a key role in the activation of G-proteins. Therefore, GPSM2 is a critical factor for the stability of cell division. Some recent studies have shown that GPSM2 messenger RNA (mRNA) is overexpressed and plays a positive role in the development of certain tumors, such as liver cancer, pancreatic cancer, breast cancer. It also plays a role in neuroblast division and in the development of normal hearing. Mutations in GPSM2 are associated with autosomal recessive nonsyndromic deafness (DFNB82), which is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GPSM2 antibody 26798-1-AP | Download protocol |
| IHC protocol for GPSM2 antibody 26798-1-AP | Download protocol |
| WB protocol for GPSM2 antibody 26798-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Mol Med Comprehensive Analysis of GPSM2: From Pan-Cancer Analysis to Experimental Validation | ||
Nan Fang Yi Ke Da Xue Xue Bao [GPSM2 is highly expressed in gastric cancer to affect patient prognosis by promoting tumor cell proliferation] |
