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验证数据展示

  • IHC staining of human gliomas using 12159-1-AP

    IHC staining of human gliomas using 12159-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12159-1-AP (GPRASP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 12159-1-AP

    WB analysis of HEK-293 using 12159-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12159-1-AP (GPRASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

GPRASP2 Polyclonal antibody

GPRASP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12159-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GASP 2, GASP2, GPRASP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12159-1-AP targets GPRASP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 GPRASP2 fusion protein Ag2800 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor associated sorting protein 2
别名 GASP 2, GASP2, GPRASP2
计算分子量 838 aa, 94 kDa
观测分子量 94 kDa
GenBank蛋白编号BC013576
基因名称 GPRASP2
Gene ID (NCBI) 114928
RRIDAB_2232653
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96D09
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPRASP2 antibody 12159-1-APDownload protocol
IHC protocol for GPRASP2 antibody 12159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Abnormal mGluR-mediated synaptic plasticity and autism-like behaviours in Gprasp2 mutant mice.

Authors - Mohamed Edfawy
  • KO Validated
View Article
mouseIHC

J Med Genet

GPRASP2, a novel causative gene mutated in an X-linked recessive syndromic hearing loss.

Authors - Guangqian Xing
View Article