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验证数据展示

  • IHC staining of mouse ovary using 16756-1-AP

    IHC staining of mouse ovary using 16756-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16756-1-AP (GPR97 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse ovary using 16756-1-AP

    IHC staining of mouse ovary using 16756-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16756-1-AP (GPR97 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR97 Polyclonal antibody

GPR97 Polyclonal Antibody for IHC, ELISA
Cat No. 16756-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

ADGRG3, Adhesion G protein-coupled receptor G3, G protein coupled receptor 97, GPR 97, G-protein coupled receptor 97

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16756-1-AP targets GPR97 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10272

Product name: Recombinant human GPR97 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-274 aa of BC064508

Sequence: KPTEGPRNTCLGSNNMYDIFNLNDKALCFTKCRQSGSDSCNVENLQRYWLNYEAHLMKEGLTQKVNTPFLKALVQNLSTNTAEDFYFSLEPSQVPRQVMKDEDKPPDRVRLPKSLFRSLPGNRSVVRLAVTILDIGPGTLFKGPRLGLGDGSGVLNNRLVGLSVGQMHVTKLAEPLEIVFSHQRPPPNMTLTCVFWDVTKGTTGDWSSEGCSTEVRPEGTVCCCDHLTFFALLLRPTLDQSTVHILTRISQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 97
别名 ADGRG3, Adhesion G protein-coupled receptor G3, G protein coupled receptor 97, GPR 97, G-protein coupled receptor 97
计算分子量 549 aa, 61 kDa
GenBank蛋白编号BC064508
基因名称 GPR97
Gene ID (NCBI) 222487
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86Y34
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR97, also known as adhesion G-protein-coupled receptor G3 (ADGRG3), belongs to the GPCR family. GPR97 is involved in acute kidney injury (AKI) progression, with GPR97 deficiency reducing renal injury and inflammation in AKI models (PMID: 30559745).

实验方案

Product Specific Protocols
IHC protocol for GPR97 antibody 16756-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols