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验证数据展示

  • IHC staining of mouse bladder using 25999-1-AP

    IHC staining of mouse bladder using 25999-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 25999-1-AP (GPR87 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse bladder using 25999-1-AP

    IHC staining of mouse bladder using 25999-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 25999-1-AP (GPR87 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR87 Polyclonal antibody

GPR87 Polyclonal Antibody for IHC, ELISA
Cat No. 25999-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

FKSG78, G protein coupled receptor 87, G protein coupled receptor 95, GPR87, GPR95, KPG_002

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25999-1-AP targets GPR87 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag23214

Product name: Recombinant human GPR87 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-47 aa of BC132888

Sequence: MGFNLTLAKLPNNELHGQESHNSGNRSDGPGKNTTLHNEFDTIVLPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 87
别名 FKSG78, G protein coupled receptor 87, G protein coupled receptor 95, GPR87, GPR95, KPG_002
GenBank蛋白编号BC132888
基因名称 GPR87
Gene ID (NCBI) 53836
RRIDAB_3085833
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BY21
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

G Protein-Coupled Receptor 87 (GPR87) is a GPCR, and it has been demonstrated to regulate the progression of several tumors (PMID:18183596). Studied reported that GPR87 promotes PDA (Pancreatic ductal adenocarcinoma) proliferation, angiogenesis, gemcitabine resistance, and tumorigenicity through activating the nuclear factor-kB (NF-kB) pathway (PMID:32405536). GPR87 is an independent prognostic factor for bladder cancer intravesical recurrence; it promotes bladder cancer cell proliferation and inhibits apoptosis. In addition, GPR87 was deorphanized and shown to be a lysophosphatidic acid (LPA) receptor (PMID:23752273).

实验方案

Product Specific Protocols
IHC protocol for GPR87 antibody 25999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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