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GPR50 Polyclonal antibody
GPR50 Polyclonal Antibody for FC, IF-P, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC, IF-P, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse brain tissue, SH-SY5Y cells |
Positive FC detected in | K-562 cells |
推荐稀释比
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19762-1-AP targets GPR50 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | FC, IF-P, IHC, ELISA |
Cited Applications | WB, IP, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | G protein-coupled receptor 50 |
Synonyms | G protein coupled receptor 50, GPR50, H9, Mel1c, Melatonin related receptor |
Calculated Molecular Weight | 67 kDa |
GenBank Accession Number | NM_004224 |
Gene Symbol | GPR50 |
Gene ID (NCBI) | 9248 |
RRID | AB_2878603 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13585 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IHC protocol for GPR50 antibody 19762-1-AP | Download protocol |
IF protocol for GPR50 antibody 19762-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nat Cell Biol Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins. | ||
Transl Stroke Res Melatonin Offers Dual-Phase Protection to Brain Vessel Endothelial Cells in Prolonged Cerebral Ischemia-Recanalization Through Ameliorating ER Stress and Resolving Refractory Stress Granule | ||
Front Pharmacol An Improved Genetically Encoded Fluorescent cAMP Indicator for Sensitive cAMP Imaging and Fast Drug Screening.
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Am J Chin Med Tetramethylpyrazine Attenuated Sevoflurane-Induced Neurotoxicity by Enhancing Autophagy through GPR50/CREB Pathway in SH-SY5Y Cells.
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FEBS Open Bio Orphan receptor GPR50 attenuates inflammation and insulin signaling in 3T3-L1 preadipocytes
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