验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, unboiled mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84587-1-RR targets GPR45 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 45 |
| 别名 | PSP24(ALPHA), PSP24 alpha, PSP24 1, PSP24, G protein coupled receptor 45 |
| 计算分子量 | 370 aa, 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC066879 |
| 基因名称 | GPR45 |
| Gene ID (NCBI) | 11250 |
| RRID | AB_3672083 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9Y5Y3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR45 (G-protein coupled receptor 45) is a member of the G protein-coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. GPR45 may function in the central nervous system.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR45 antibody 84587-1-RR | Download protocol |
| IHC protocol for GPR45 antibody 84587-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |