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验证数据展示

  • WB analysis using 22149-1-AP

    WB analysis using 22149-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22149-1-AP (GPR34 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 22149-1-AP

    IHC staining of mouse brain using 22149-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22149-1-AP (GPR34 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR34 Polyclonal antibody

GPR34 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22149-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

G protein coupled receptor 34, Probable G-protein coupled receptor 34

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22149-1-AP targets GPR34 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17742

Product name: Recombinant human GPR34 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 267-381 aa of BC020678

Sequence: NSFIVLIIFTICFVPYHAFRFIYISSQLNVSSCYWKEIVHKTNEIMLVLSSFNSCLDPVMYFLMSSNIRKIMCQLLFRRFQGEPSRSESTSEFKPGYSLHDTSVAVKIQSSSKST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 34
别名 G protein coupled receptor 34, Probable G-protein coupled receptor 34
计算分子量 381 aa, 44 kDa
观测分子量38 kDa
GenBank蛋白编号BC020678
基因名称 GPR34
Gene ID (NCBI) 2857
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPC5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

G protein-coupled receptor 34 (GPR34) is a 7-transmembrane orphan receptor, belonging to the GPCR family, which affects multiple biological and physiological functions, such as cell proliferation and motility, immune infiltration, gene transcription.

实验方案

Product Specific Protocols
WB protocol for GPR34 antibody 22149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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