验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22149-1-AP targets GPR34 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag17742 Product name: Recombinant human GPR34 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 267-381 aa of BC020678 Sequence: NSFIVLIIFTICFVPYHAFRFIYISSQLNVSSCYWKEIVHKTNEIMLVLSSFNSCLDPVMYFLMSSNIRKIMCQLLFRRFQGEPSRSESTSEFKPGYSLHDTSVAVKIQSSSKST 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 34 |
| 别名 | G protein coupled receptor 34, Probable G-protein coupled receptor 34 |
| 计算分子量 | 381 aa, 44 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC020678 |
| 基因名称 | GPR34 |
| Gene ID (NCBI) | 2857 |
| RRID | AB_3742067 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UPC5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
G protein-coupled receptor 34 (GPR34) is a 7-transmembrane orphan receptor, belonging to the GPCR family, which affects multiple biological and physiological functions, such as cell proliferation and motility, immune infiltration, gene transcription. GPR34 is indeed highly expressed on ILC1-like cells in the tumors of patients with colon adenocarcinoma, head and neck cancer, and liver cancer (PMID: 39358444).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPR34 antibody 22149-1-AP | Download protocol |
| WB protocol for GPR34 antibody 22149-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
