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验证数据展示

  • IHC staining of mouse brain using 20847-1-AP

    IHC staining of mouse brain using 20847-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20847-1-AP (GPR3 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20847-1-AP

    IHC staining of mouse brain using 20847-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20847-1-AP (GPR3 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR3 Polyclonal antibody

GPR3 Polyclonal Antibody for IHC, ELISA
Cat No. 20847-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA and More (1)

别名

ACCA, ACCA orphan receptor, G protein coupled receptor 3, GPR3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20847-1-AP targets GPR3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
免疫原

CatNo: Ag14257

Product name: Recombinant human GPR3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 271-330 aa of BC032702

Sequence: DAHSPPLYTYLTLLPATYNSMINPIIYAFRNQDVQKVLWAVCCCCSSSKIPFRSRSPSDV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 3
别名 ACCA, ACCA orphan receptor, G protein coupled receptor 3, GPR3
计算分子量 330 aa, 35 kDa
GenBank蛋白编号BC032702
基因名称 GPR3
Gene ID (NCBI) 2827
RRIDAB_2878749
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46089
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR3 (G protein-coupled receptor 3) was first cloned from a mouse cDNA library in 1993 before being cloned from human genomic libraries by several independent groups (PMID: 29941868). GPR3 mRNA is broadly expressed in neurons in various brain regions, including the cortex, thalamus, hypothalamus, amygdala, hippocampus, pituitary, and cerebellum (PMID: 22207171). Moreover, the GPR3 protein is overexpressed in neurons in post-mortem brain tissue sections from individuals afflicted by Alzheimer's disease (PMID: 19213921).

实验方案

Product Specific Protocols
IHC protocol for GPR3 antibody 20847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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