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验证数据展示

  • IHC staining of mouse brain using 20847-1-AP

    IHC staining of mouse brain using 20847-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20847-1-AP (GPR3 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20847-1-AP

    IHC staining of mouse brain using 20847-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20847-1-AP (GPR3 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR3 Polyclonal antibody

GPR3 Polyclonal Antibody for IHC, ELISA
Cat No. 20847-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA and More (1)

别名

ACCA, ACCA orphan receptor, G protein coupled receptor 3, GPR3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20847-1-AP targets GPR3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
免疫原 GPR3 fusion protein Ag14257 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 3
别名 ACCA, ACCA orphan receptor, G protein coupled receptor 3, GPR3
计算分子量 330 aa, 35 kDa
GenBank蛋白编号BC032702
基因名称 GPR3
Gene ID (NCBI) 2827
RRIDAB_2878749
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46089
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR3 (G protein-coupled receptor 3) was first cloned from a mouse cDNA library in 1993 before being cloned from human genomic libraries by several independent groups (PMID: 29941868). GPR3 mRNA is broadly expressed in neurons in various brain regions, including the cortex, thalamus, hypothalamus, amygdala, hippocampus, pituitary, and cerebellum (PMID: 22207171). Moreover, the GPR3 protein is overexpressed in neurons in post-mortem brain tissue sections from individuals afflicted by Alzheimer's disease (PMID: 19213921).

实验方案

Product Specific Protocols
IHC protocol for GPR3 antibody 20847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

iScience

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Authors - Kayla Sompel
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WB,IHC

Oral Oncol

The circadian clock gene, BMAL1, promotes radiosensitization in nasopharyngeal carcinoma by inhibiting the epithelial-to-mesenchymal transition via the TGF-β1/Smads/Snail1 axis

Authors - Yuxin Li
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