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验证数据展示

  • FC experiment of SH-SY5Y using 19951-1-AP

    FC experiment of SH-SY5Y using 19951-1-AP

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug GPR22 Polyclonal antibody (19951-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • WB analysis of mouse heart using 19951-1-AP

    WB analysis of mouse heart using 19951-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 19951-1-AP (GPR22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 19951-1-AP

    IHC staining of human testis using 19951-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19951-1-AP (GPR22 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 19951-1-AP

    IHC staining of human testis using 19951-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19951-1-AP (GPR22 Antibody) at dilution of 1:100 (under 40x lens).

GPR22 Polyclonal antibody

GPR22 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19951-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

G protein coupled receptor 22, G-protein coupled receptor 22

缓冲液配方:  PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19951-1-AP targets GPR22 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 22
别名 G protein coupled receptor 22, G-protein coupled receptor 22
计算分子量 49 kDa
观测分子量50-55 kDa
GenBank蛋白编号NM_005295
基因名称 GPR22
Gene ID (NCBI) 2845
RRIDAB_10644129
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99680
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR22 Belongs to the G-protein coupled receptor 1 family. GPR22 is an Orphan receptor. The antibody recognize the C-term of GPR22.

实验方案

Product Specific Protocols
WB protocol for GPR22 antibody 19951-1-APDownload protocol
IHC protocol for GPR22 antibody 19951-1-APDownload protocol
FC protocol for GPR22 antibody 19951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

BMC Cardiovasc Disord

Cardiomyocyte-specific overexpression of GPR22 ameliorates cardiac injury in mice with acute myocardial infarction.

Authors - Chin-Chuan Chang
View Article
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