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验证数据展示

  • WB analysis using 87492-4-RR

    WB analysis using 87492-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87492-4-RR (GPR183 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of Raji using 87492-4-RR

    IF Staining of Raji using 87492-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using GPR183 antibody (87492-4-RR, Clone: 260283A6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of Daudi using 87492-4-RR

    IF Staining of Daudi using 87492-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using GPR183 antibody (87492-4-RR, Clone: 260283A6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GPR183 Recombinant monoclonal antibody

GPR183 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87492-4-RR

产品说明书

CloneNo. 260283A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

EBI2, GPR183/EBI2, CDw381, EBV-induced G-protein coupled receptor 2, Epstein-Barr virus-induced G-protein coupled receptor 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells, Daudi cells, Fetal human brain tissue
Positive IF/ICC detected inRaji cells, Daudi cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87492-4-RR targets GPR183 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 G protein-coupled receptor 183
别名 EBI2, GPR183/EBI2, CDw381, EBV-induced G-protein coupled receptor 2, Epstein-Barr virus-induced G-protein coupled receptor 2
计算分子量 361 aa, 41 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC020752
基因名称 EBI2
Gene ID (NCBI) 1880
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP32249
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

G-protein-coupled receptor 183, also known as Epstein-Barr virus-induced gene 2 (EBI2), was initially identified in B cells and is the most upregulated gene after infection with Epstein-Barr virus (PMID:37353188). GPR183 is known to play a key role in the development of antibody responses in secondary lymphoid organs (PMID:23473835).

实验方案

Product Specific Protocols
IF protocol for GPR183 antibody 87492-4-RRDownload protocol
WB protocol for GPR183 antibody 87492-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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