验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32243-1-AP targets GPR183/EBI2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag36765 Product name: Recombinant human GPR183 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 313-361 aa of BC020752 Sequence: KGYKRKVMRMLKRQVSVSISSAVKSAPEENSREMTETQMMIHSKSSNGK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 183 |
| 别名 | EBI2, GPR183, EBV-induced G-protein coupled receptor 2, Epstein-Barr virus-induced G-protein coupled receptor 2, G protein coupled receptor 183 |
| 计算分子量 | 361 aa, 41 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC020752 |
| 基因名称 | EBI2 |
| Gene ID (NCBI) | 1880 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P32249 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
G-protein-coupled receptor 183, also known as Epstein-Barr virus-induced gene 2 (EBI2), was initially identified in B cells and is the most upregulated gene after infection with Epstein-Barr virus (PMID:37353188). GPR183 is known to play a key role in the development of antibody responses in secondary lymphoid organs (PMID:23473835).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR183/EBI2 antibody 32243-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
