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GPR183/EBI2 Polyclonal antibody

GPR183/EBI2 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 12377-1-AP

Print datasheet

Synonyms

EBI2, G protein coupled receptor 183, GP183, GPR183

验证数据展示

  • WB analysis of Jurkat using 12377-1-AP

    WB analysis of Jurkat using 12377-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12377-1-AP (GPR183/EBI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 12377-1-AP

    IP experiment of mouse lung using 12377-1-AP

    IP result of anti-GPR183/EBI2 (IP:12377-1-AP, 4ug; Detection:12377-1-AP 1:500) with mouse lung tissue lysate 4000ug.

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, mouse lung tissue, Raji cells
Positive IP detected inmouse lung tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

12377-1-AP targets GPR183/EBI2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen GPR183/EBI2 fusion protein Ag3035 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 183
Synonyms EBI2, G protein coupled receptor 183, GP183, GPR183
Calculated Molecular Weight 361 aa, 41 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC020752
Gene Symbol GPR183
Gene ID (NCBI) 1880
RRIDAB_2114261
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32249
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPR183/EBI2 antibody 12377-1-APDownload protocol
IP protocol for GPR183/EBI2 antibody 12377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Allergy Clin Immunol

Chronic rhinosinusitis with nasal polyps is characterized by B-cell inflammation and EBV-induced protein 2 expression.

Authors - Hulse Kathryn E KE
View Article
humanWB

J Pharmacol Exp Ther

7α,25-Dihydroxycholesterol Suppresses Hepatocellular Steatosis through GPR183/EBI2 in Mouse and Human Hepatocytes.

Authors - Jin Huang
View Article
mouseWB

Neurotox Res

Phoenixin-20 Ameliorates Lipopolysaccharide-Induced Activation of Microglial NLRP3 Inflammasome.

Authors - Xiangliang Zeng
View Article
mouseWB

Eur J Pharmacol

Activation of G-protein-coupled receptor 183 initiates inflammatory pain via macrophage CCL22 secretion

Authors - Zhenhua Qi
View Article