验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16691-1-AP targets GPR180 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag10052 Product name: Recombinant human GPR180 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 18-170 aa of BC052243 Sequence: QGSQGKTLRGSFSSTAAQDAQGQRIGHFEFHGDHALLCVRINNIAVAVGKEAKLYLFQAQEWLKLQQSSHGYSCSEKLSKAQLTMTMNQTEHNLTVSQIPSPQTWHVFYADKYTCQDDKENSQVEDIPFEMVLLNPDAEGNPFDHFSAGESGL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 180 |
| 别名 | Integral membrane protein GPR180, Intimal thickness-related receptor, ITR |
| 计算分子量 | 440 aa, 49 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC052243 |
| 基因名称 | GPR180 |
| Gene ID (NCBI) | 160897 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86V85 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR180 is an orphan GPCR, also known as the Endothelial Thickness-Related Receptor (ITR), and is a member of the GOLDS domain seven transmembrane helix (GOST) protein family. This receptor was initially discovered to promote the proliferation of vascular smooth muscle cells, leading to subsequent thickening of the vascular intima. GPR180 interacts with CTHRC1 to regulate the TGFβ signaling pathway, influencing energy metabolism and thermogenic function ( PMID: 34880217).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR180 antibody 16691-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
