验证数据展示
产品信息
33499-1-PBS targets GPR175 in WB, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, Indirect ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag39379 Product name: Recombinant human GPR175 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 286-373 aa of BC050711 Sequence: SEPKILFSYKCQVDETEEPDVHLPQPYAVARREGLEAAGAAGASAASYSSTQFDSAGGVAYLDDIASMPCHTGSINSTDSERWKAINA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 175 |
| 别名 | Integral membrane protein GPR175, PP6566, TMEM227, TPRA1, TPRA40 |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC050711 |
| 基因名称 | GPR175 |
| Gene ID (NCBI) | 131601 |
| RRID | AB_3742992 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q86W33 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
GPR175, also known as TPRA1 or TPRA40, is a transmembrane protein. GPR175 was first cloned as a GLP-1 receptor homolog in 3T3-L1 adipocytes and is also expressed in tissues that require Hh signaling during development, including the heart, brain, lung, pancreas, and muscle. Endogenous GPR175 localizes to cilia in a Hh-dependent manner, where it can interact with (constitutively) ciliary Gαi1 to inhibit the local production of cAMP by adenylyl cyclase. The calculated molecular weight of GPR175 is 41 kDa. With glycosylation modification, the molecular weight of GPR175 will be migrated to 55 kDa. (PMID: 26451044)
