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验证数据展示

  • WB analysis using 20791-1-AP

    WB analysis using 20791-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20791-1-AP (GPR172B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 20791-1-AP

    IHC staining of mouse testis using 20791-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20791-1-AP (GPR172B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR172B Polyclonal antibody

GPR172B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20791-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

RFT1, Protein GPR172B, PERV A receptor 2, PAR2, hRFT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, mouse brain tissue, mouse small intestine tissue, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20791-1-AP targets GPR172B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 GPR172B fusion protein Ag14525 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 172B
别名 RFT1, Protein GPR172B, PERV A receptor 2, PAR2, hRFT1
计算分子量 448 aa, 46 kDa
观测分子量50 kDa
GenBank蛋白编号BC060810
基因名称 GPR172B
Gene ID (NCBI) 55065
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWF4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR172B, also known as solute carrier family 52 member 1 (SLC52A1) or riboflavin transporter 1 (RFT1), is a G protein-coupled receptor (GPCR) that plays a significant role in the transport of riboflavin, a vital component for the production of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN). GPR172B plays a role in cellular aging. Knockdown of GPR172B promotes senescence induced by DNA damage in both tumor and normal cells. The anti-senescence effect of GPR172B is dependent on its riboflavin transport activity, which leads to the activation of mitochondrial membrane potential (MMP) mediated by mitochondrial electron transport chain complex II, ultimately inhibiting the AMPK-p53 pathway, a central mediator of senescence-associated with mitochondrial dysfunction.

实验方案

Product Specific Protocols
WB protocol for GPR172B antibody 20791-1-APDownload protocol
IHC protocol for GPR172B antibody 20791-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols