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GPR17 Polyclonal antibody

GPR17 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, WB,ELISA

Conjugate

Unconjugated

Cat No : 13416-1-AP

Print datasheet

Synonyms

G protein coupled receptor 17, GPR17, P2Y like receptor, R12, UDP/CysLT receptor

验证数据展示

  • WB analysis of human plasma using 13416-1-AP

    WB analysis of human plasma using 13416-1-AP

    human serum were subjected to SDS PAGE followed by western blot with 13416-1-AP (GPR17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 13416-1-AP

    FC experiment of SH-SY5Y using 13416-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug GPR17 antibody (13416-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inhuman serum tissue
Positive FC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13416-1-AP targets GPR17 in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen GPR17 fusion protein Ag4106 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 17
Synonyms G protein coupled receptor 17, GPR17, P2Y like receptor, R12, UDP/CysLT receptor
Calculated Molecular Weight 367 aa, 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC031653
Gene Symbol GPR17
Gene ID (NCBI) 2840
RRIDAB_2279341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13304
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPR17 antibody 13416-1-APDownload protocol
FC protocol for GPR17 antibody 13416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols