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验证数据展示

  • WB analysis of Jurkat using 24878-1-AP

    WB analysis of Jurkat using 24878-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24878-1-AP (GPR153 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 24878-1-AP

    IHC staining of human liver using 24878-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24878-1-AP (GPR153 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 24878-1-AP

    IHC staining of human liver using 24878-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24878-1-AP (GPR153 Antibody) at dilution of 1:100 (under 40x lens).

GPR153 Polyclonal antibody

GPR153 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24878-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

G protein coupled receptor 153, GPR153, PGR1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24878-1-AP targets GPR153 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19279

Product name: Recombinant human GPR153 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 298-609 aa of BC068275

Sequence: WACDRYRADLKAVREKCMALMANDEESDDETSLEGGISPDLVLERSLDYGYGGDFVALDRMAKYEISALEGGLPQLYPLRPLQEDKMQYLQVPPTRRFSHDDADVWAAVPLPAFLPRWGSGEDLAALAHLVLPAGPERRRASLLAFAEDAPPSRARRRSAESLLSLRPSALDSGPRGARDSPPGSPRRRPGPGPRSASASLLPDAFALTAFECEPQALRRPPGPFPAAPAAPDGADPGEAPTPPSSAQRSPGPRPSAHSHAGSLRPGLSASWGEPGGLRAAGGGGSTSSFLSSPSESSGYATLHSDSLGSAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 153
别名 G protein coupled receptor 153, GPR153, PGR1
计算分子量 609 aa, 65 kDa
观测分子量 65 kDa
GenBank蛋白编号BC068275
基因名称 GPR153
Gene ID (NCBI) 387509
RRIDAB_2879774
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6NV75
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPR153 antibody 24878-1-APDownload protocol
IHC protocol for GPR153 antibody 24878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols