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验证数据展示

  • IHC staining of mouse testis using 25996-1-AP

    IHC staining of mouse testis using 25996-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25996-1-AP (GPR148 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 25996-1-AP

    IHC staining of mouse testis using 25996-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25996-1-AP (GPR148 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 25996-1-AP

    IHC staining of mouse testis using 25996-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25996-1-AP (GPR148 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR148 Polyclonal antibody

GPR148 Polyclonal Antibody for IHC, ELISA
Cat No. 25996-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

BTR, G protein coupled receptor 148, PGR6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25996-1-AP targets GPR148 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22948

Product name: Recombinant human GPR148 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-95 aa of BC105013

Sequence: MGDELAPCPVGTTAWPALIQLISKTPCMPQAASNTSLGLGDLRVPSSMLYWLFLPSSLLAAATLAVSPLLLVTILRNQRLRQEPHYLLPANILLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 148
别名 BTR, G protein coupled receptor 148, PGR6
计算分子量 347 aa, 38 kDa
GenBank蛋白编号BC105013
基因名称 GPR148
Gene ID (NCBI) 344561
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDV2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR148, also known as BTR or PGR6, belongs to a G protein-coupled receptor (GPCR) family. It is primarily expressed in the brain and testis, suggesting a role in specific sensory functions and possibly in reproductive processes. As a GPCR, GPR148 is likely to be involved in signal transduction pathways triggered by the binding of specific ligands, such as odorants or other small molecules.

实验方案

Product Specific Protocols
IHC protocol for GPR148 antibody 25996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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