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验证数据展示

  • IHC staining of human kidney using 28096-1-AP

    IHC staining of human kidney using 28096-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28096-1-AP (GPR141 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 28096-1-AP

    IHC staining of human kidney using 28096-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28096-1-AP (GPR141 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28096-1-AP

    IHC staining of mouse brain using 28096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28096-1-AP (GPR141 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR141 Polyclonal antibody

GPR141 Polyclonal Antibody for IHC, ELISA
Cat No. 28096-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

IHC, ELISA

别名

G protein coupled receptor 141, GPR141, PGR13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28096-1-AP targets GPR141 in IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 GPR141 fusion protein Ag23129 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 141
别名 G protein coupled receptor 141, GPR141, PGR13
计算分子量 305 aa, 35 kDa
GenBank蛋白编号BC120951
基因名称 GPR141
Gene ID (NCBI) 353345
RRIDAB_3086028
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z602
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR141 (G-protein-coupled receptor 141) is a class A orphan receptor molecule of the rhodopsin family. Increased GPR141 expression enhances the migratory behavior of breast cancer, driving oncogenic pathways both in vitro and in vivo through activation of epithelial to mesenchymal transition (EMT), oncogenic mediators, and regulation of p-mTOR/p53 signaling (PMID: 37204251).

实验方案

Product Specific Protocols
IHC protocol for GPR141 antibody 28096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols