验证数据展示
经过测试的应用
| Positive IHC detected in | mouse kidney tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
17774-1-AP targets GPR126 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12195 Product name: Recombinant human GPR126 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 45-399 aa of BC075798 Sequence: LSNPSGTFTSPCYPNDYPNSQACMWTLRAPTGYIIQITFNDFDIEEAPNCIYDSLSLDNGESQTKFCGATAKGLSFNSSANEMHVSFSSDFSIQKKGFNASYIRVAVSLRNQKVILPQTSDAYQVSVAKSISIPELSAFTLCFEATKVGHEDSDWTAFSYSNASFTQLLSFGKAKSGYFLSISDSQCLLNNALPVKEKEDIFAESFEQLCLVWNNSLGSIGVNFKRNYETVPCDSTISKVIPGNGKLLLGSNQNEIVSLKGDIYNFRLWNFTMNAKILSNLSCNVKGNVVDWQNDFWNIPNLALKAESNLSCGSYLIPLPAAELASCADLGTLCQDGIIYRISVVIQNILRHPEV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 126 |
| 别名 | ADGRG6, APG1, DREG, G protein coupled receptor 126, GPR126, PS1TP2, VIGR |
| 计算分子量 | 1222 aa, 137 kDa |
| GenBank蛋白编号 | BC075798 |
| 基因名称 | GPR126 |
| Gene ID (NCBI) | 57211 |
| RRID | AB_2878439 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86SQ4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR126 (G protein-coupled receptor 126) is a member of the adhesion GPCR family. GPR126 is all widely expressed on stromal cells (PMID: 25713288). It is essential for normal differentiation of promyelinating Schwann cells and for normal myelination of axons (PMID: 24227709). GPR126 regulates neural, cardiac and ear development by G-protein- and/or N-terminus-dependent signaling (PMID: 24082093, PMID: 24067352). GPR126 also stimulates VEGF signaling and angiogenesis by modulating VEGF receptor 2 expression through STAT5 and GATA2 in endothelial cells.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPR126 antibody 17774-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
