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GPR125 Polyclonal antibody

GPR125 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 11912-1-AP

Print datasheet

Synonyms

G protein coupled receptor 125, GPR125, PGR21, TEM5L

验证数据展示

  • WB analysis of pig brain using 11912-1-AP

    WB analysis of pig brain using 11912-1-AP

    pig brain tissue were subjected to SDS PAGE followed by western blot with 11912-1-AP (GPR125 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 11912-1-AP

    IHC staining of mouse brain using 11912-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11912-1-AP (GPR125 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11912-1-AP

    IHC staining of mouse brain using 11912-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11912-1-AP (GPR125 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inpig brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11912-1-AP targets GPR125 in WB, IHC, ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, pig, mouse
Immunogen GPR125 fusion protein Ag2513 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 125
Synonyms G protein coupled receptor 125, GPR125, PGR21, TEM5L
Calculated Molecular Weight 1321 aa, 146 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC026009
Gene Symbol GPR125
Gene ID (NCBI) 166647
RRIDAB_2877804
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWK6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPR125 antibody 11912-1-APDownload protocol
IHC protocol for GPR125 antibody 11912-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols