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验证数据展示

  • IHC staining of mouse small intestine using 20050-1-AP

    IHC staining of mouse small intestine using 20050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20050-1-AP (GPR124 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 20050-1-AP

    IHC staining of mouse lung using 20050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20050-1-AP (GPR124 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR124 Polyclonal antibody

GPR124 Polyclonal Antibody for IHC, ELISA
Cat No. 20050-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

Tumor endothelial marker 5, TEM5, KIAA1531, G-protein coupled receptor 124, G protein coupled receptor 124

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse small intestine tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20050-1-AP targets GPR124 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 124
别名 Tumor endothelial marker 5, TEM5, KIAA1531, G-protein coupled receptor 124, G protein coupled receptor 124
计算分子量 143 kDa
GenBank蛋白编号NM_032777
基因名称 GPR124
Gene ID (NCBI) 25960
RRIDAB_3669320
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PE1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR124, also named KIAA1531 and TEM5, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. GPR124 is an Orphan receptor. GPR124 is a Tumor endothelial marker (TEM) that is significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. GPR124 is a member of the adhesion family of G protein-coupled receptors and is localized on the surface of endothelial cells. GPR124 is a seven-pass transmembrane receptor, unlike TEM1, TEM7, and TEM8 which span the membrane once. Proteolytically processed soluble GPR124 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans. The antibody recognizes near the C-term of GPR124. The antibody can't recognize the isoform3 of GPR124.

实验方案

Product Specific Protocols
IHC protocol for GPR124 antibody 20050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols